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WHAT IS PROTEINASE K MADE OF? Proteinase K is known enzyme belonging to the class of serine proteases that cleave adjacent to the carboxylic group peptide bonds of aromatic and aliphatic amino acids. It's also exhibited a very broad cleavage specificity. The enzyme was discovered in extracts of the fungus Engyodontium album. HOW DOES PROTEINASE K WORK? In molecular biology, main application of proteinase K is to digest proteins during nucleic acids purification in order to remove protein contaminants: - Proteinase K quickly inactivates nucleases (enzymes that digest nucleic acids) that degrade DNA or RNA molecules during the cleansing. - Proteinase K is activated by calcium. It does not affect the activity of the proteinase, but protects again autolysis, improves thermal stability, and regulates substrate binding capacity. To increase the stability of Proteinase K, thereby enhancing its action, Urea as well as SDS (sodium dodecyl sulfate) or elevated temperature at 37-60C is commonly used. Inactivation of Proteinase K is occurring at temperature above 65C. WHAT IS THE PURPOSE OF PROTEINASES? Proteinase K is used to break down proteins in cell lysates (tissues, cell culture cells) and to release nucleic acids due to its action described above. Proteinase K application purpose: DNA isolation from various tissues; Removal of DNAses and RNAses: Proteinase K and RNases can act together in the lysis buffer to degrades contaminating RNAs and proteins;

Are you attempting to eat healthy in order to prepare for your summer beach body? When it comes to achieving optimal body composition, following a healthy diet can be challenging. It's very easy to eat too many calories while eating foods that society considers to be healthy. It's important to remember that calorie counting isn't the only way to lose weight, and it won't be a long-term strategy either. So, here are some practical suggestions for maintaining a healthy weight while also toning your muscles. 1. LEARN ABOUT THE PROCESS OF PROTEIN-RICH FOODS Protein-rich foods are the most effective at curbing hunger and are essential for maintaining a healthy weight and body composition. An added benefit of caloric restriction is that it triggers the body's catabolic process, which results in the loss of both lean muscle and fat. Having a lower metabolic rate is one of the main causes of rebound fat gain when you lose a lot of muscle. Taking in a lot of protein throughout the day has been shown to help counteract some muscle loss and keep your metabolism going strong. As a bonus, high-quality protein can help control blood sugar and insulin levels, reducing the desire for sweets.

protein helps your hair grow and lose weight -Researchers have showed how hair growth activated fat tissue growth within the skin below the follicle may lead to the development of a cream to dissolve fat. particularly, the protein that activated follicle growth was shown to conjointly inhibit fat production

Global pharmaceutical and biotechnology company AstraZeneca has signed an agreement with Scorpion Therapeutics to discover, develop and commercialize precision medicines against cancer proteins. The collaboration, announced today (January 13), will focus on a class of proteins called transcription factors, which can regulate important cellular processes including cell growth and survival. Many transcription factors have been identified earlier, but were considered 'undruggable' using conventional drug discovery approaches, the company said in a statement. Susan Galbraith, executive vice president, Oncology R&D, AstraZeneca, said: "Unlocking potentially transformative biology is pivotal for delivering the next wave of cancer treatments. "Scorpion's innovative platform is a strong strategic fit as we explore a range of new modalities across our broad drug discovery toolbox with promise to disrupt the activity of these highly-validated cancer targets."

GlaxoSmithKline (GSK) says initial tests of the antibody-based Covid-19 therapy it is developing with US partner Vir suggest the drug is effective against the Omicron variant. A GSK statement on Thursday (December 2) said that lab tests and a study on hamsters have demonstrated the sotrovimab antibody cocktail to work against viruses that were bio-engineered to carry a number of hallmark mutations of the new variant. The tests are continuing to confirm the results against all of the Omicron mutations, with an update expected by the end of the year, it added. The antibody is designed to latch on to the spike protein on the surface of the coronavirus, but Omicron has been found to have an unusually high amount of mutations on that protein. "Sotrovimab was deliberately designed with a mutating virus in mind," said Vir chief executive George Scangos, adding that the drug was targeting a region of the spike protein that was highly unlikely to mutate.

British drugmaker GSK said on Tuesday (December 7) its antibody-based Covid-19 therapy with US partner Vir Biotechnology is effective against all mutations of the new Omicron coronavirus variant, citing new data from early-stage studies. The data, yet to be published in a peer-reviewed medical journal, shows that the companies' treatment, sotrovimab, is effective against all 37 identified mutations to date in the spike protein, GSK said in a statement. Last week, another pre-clinical data showed that the drug had worked against key mutations of the Omicron variant. Sotrovimab is designed to latch on to the spike protein on the surface of the coronavirus, but Omicron has been found to have an unusually high number of mutations on that protein. "These pre-clinical data demonstrate the potential for our monoclonal antibody to be effective against the latest variant, Omicron, plus all other variants of concern defined to date by the WHO," GSK chief scientific officer Hal Barron said.

The first big breakthrough in 30 years of Alzheimer's research is providing momentum for clinical trials of "cocktail" treatments targeting the two hallmark proteins associated with the mind-robbing disease, according to interviews with researchers and pharmaceutical executives. Drugmakers Eisai and Biogen reported in September that their therapy lecanemab could slow progress of the disease by 27% over 18 months compared with a placebo. The finding validates the theory that clearing the amyloid protein that forms clumps in the brains of Alzheimer's patients could slow or halt the disease and has strengthened the support from some scientists for simultaneously targeting another notorious protein linked to Alzheimer's: tau. Eisai and Biogen are scheduled to present full data from their lecanemab study on Tuesday at the Clinical Trials on Alzheimer's Disease conference in San Francisco. The U.S. Food and Drug Administration is expected to make a decision by early January on the companies' application for accelerated approval. If approved on an accelerated basis, the companies said they would immediately apply for full U.S. regulatory approval which could help secure Medicare coverage.

We cannot deny the benefits and uses of regenerative medicines as they have been so in demand in recent times. One such effective medicine with a lot of potential health benefits is BPS-157. The peptide is thought to be extremely beneficial for those struggling with various neurological or health issues. Another medicine with the same beneficial effects is the Semax peptide. Well, the benefits of both these peptide-based medicines and their other aspects will be discussed in detail in this blog along with answering your question about the BPC-157 buy, Semax effects, and polypeptide. ALL ABOUT BPC-157: BPC-157, a pentadecapeptide consisting of 15 amino acids, is a synthetic peptide that is known for its positive effects on the human body. The peptide is derived from human gastric juice. A protein is present in the human gastric juice through which this peptide is derived and synthesized. It is called a Body Protection Protein as it exerts several therapeutic effects on the human body. Not only have health benefits or therapeutic effects, the peptide has healing and regeneration powers. It also helps in the preventing formation of gastric ulcers. This peptide is of great interest to researchers due to its numerous positive effects and benefits. DOSING LEVELS OF BPC-157: Not much about the dosing levels of BPC-157 has been researched yet but always try to concern the relevant persons before taking the medicine. The dosage of BPC-157 depends on the health and body conditions of the person taking it along with other related factors.

Whether you believe it or not, following a vegan diet can be quite eventful for your body. For instance, it can help you lose weight, get a better body structure, and so on. However, is that all? You can get that by following a thorough exercise plan as well, no? Well, yes, you can. Nevertheless, following a vegan diet plan can help you out in more than a single manner. Please keep reading this article till the end to know more about it. WHAT IS A VEGAN DIET? As a vegan, you can eat dairy food, including cheese and milk, without any boundaries. If you want to get a little bit of protein, you may consume eggs (boiled or in a gravy) too. Also, yes, plants are going to be an integral part of your diet plan too. However, you won't be able to eat meat anymore. So, no chicken, mutton, or beef for the rest of your life. Instead, you will need to focus solely on plant-based protein. BENEFITS OF FOLLOWING A VEGAN DIET A plant-based diet structure can be highly beneficial for your health. For example, it can - LOWER YOUR BLOOD PRESSURE. Hypertension, also known as high blood pressure, can increase your risk of a heart attack. It's also known to be a close counterpart of type-2 diabetes too. Fortunately, though, the diet you follow can make quite a massive difference here. For example, following a vegan diet can help you reduce the amount of oily food you're taking on a daily basis. This, in turn, can decrease the amount of cholesterol in your blood and make it easier for you to lose weight. And, the less obese you are, the lower your blood pressure will be.

Immunogenicity refers to the capacity of a vaccine to provoke an immune response in the body. An effective immune response is characterized by the production of specific antibodies, activation of immune cells, and the development of immunological memory. The goal is to elicit a durable and robust response that can prevent or control infections. Leveraging Viral Vector Technology Viral vectors serve as essential vehicles for delivering antigenic proteins or genetic material into target cells, triggering an immune response. Biotechnology companies have been exploring advanced strategies to optimize viral vector design, aiming to enhance immunogenicity and improve vaccine effectiveness. Vector Selection: The choice of viral vector plays a crucial role in determining immunogenicity. Different viruses have unique properties and characteristics that can impact immune responses. Biotechnology companies meticulously select viral vectors that possess the desired attributes, such as the ability to infect target cells efficiently and induce strong immune responses. Genetic Engineering: Advanced genetic engineering techniques are employed to modify viral vectors, tailoring them to specific vaccine requirements. By introducing specific antigenic proteins or genetic material, researchers can stimulate the immune system to mount a targeted response. This precise manipulation enhances the vaccine's ability to elicit a robust immune reaction. Immunomodulatory Elements: Biotechnology companies are incorporating immunomodulatory elements into viral vector designs. These elements can enhance the immune response by stimulating various components of the immune system, such as antigen-presenting cells and T cells. By activating and priming these immune cells, the vaccine can generate a more potent and sustained immune response. Adjuvants: Adjuvants are substances added to vaccines to enhance their immunogenicity. They stimulate and amplify the immune response, improving the

An experimental Alzheimer's drug developed by Eli Lilly and Co slowed cognitive decline by 35% in a late-stage trial, the company said on Wednesday, providing what experts say is the strongest evidence yet that removing sticky amyloid plaques from the brain benefits patients with the fatal disease. Lilly's drug, donanemab, met all goals of the trial, the company said. It slowed progression of Alzheimer's by 35% compared to a placebo in 1,182 people with early-stage disease whose brains had deposits of two key Alzheimer's proteins, beta amyloid as well as intermediate levels of tau, a protein linked with disease progression and brain cell death. The study also evaluated the drug in 552 patients with high levels of tau and found that when both groups were combined, donanemab slowed progression by 29% based on a commonly used scale of dementia progression known as the Clinical Dementia Rating Scale (CDR-SB). Using that scale, experts said Lilly's findings were roughly on par with Eisai Co Ltd and Biogen Inc's lecanemab, sold under the brand name Leqembi, which reduced cognitive decline by 27% in patients with early Alzheimer's in a study published last year. The results drove Lilly's shares to a record high, up more than 6% at $429.85. Dr. Ronald Petersen, an Alzheimer's researcher at Mayo Clinic, said Lilly's trial is the third to show removing amyloid from the brain slows progression of the disease, which could put to rest some lingering doubts about the benefits of drugs in the class and the amyloid-lowering theory. "It's modest, but I think it's real," he said of the benefit, "and I think it's clinically meaningful." Dr. Erik Musiek, a Washington University neurologist at Barnes-Jewish Hospital, said the efficacy looks as good or better than lecanemab.

Early detection and treatment of Alzheimer's disease require the use of dependable and cost-effective screening technologies. Researchers at Sweden's Karolinska Institutet have revealed that the level of tau, a protein that plays a vital role in the development of severe dementia, is associated with a kind of sugar molecule in the blood. The study, which is published in Alzheimer's & Dementia, can pave the way for a simple screening procedure able to predict onset ten years in advance. "The role of glycans, structures made up of sugar molecules, is a relatively unexplored field in dementia research," says the study's first author Robin Zhou, medical student and affiliated researcher at the Department of Neurobiology, Care Sciences and Society (NVS), Karolinska Institutet. "We demonstrate in our study that blood levels of glycans are altered early during the development of the disease. This could mean that we'll be able to predict the risk of Alzheimer's disease with only a blood test and a memory test." In Alzheimer's disease, the neurons of the brain die, which is thought to be a result of the abnormal accumulation of the proteins amyloid beta and tau. Clinical trials for Alzheimer's drugs show that treatment should commence early in the pathological process, before too many neurons have died, to reverse the process before it is too late.

there are numerous benefits of eating hemp seeds. they are one of the few food with a perfect ratio of fatty acids. they are a complete protein with tons of vitamins and minerals.

there are numerous benefits of eating hemp seeds. they are one of the few food with a perfect ratio of fatty acids. they are a complete protein with tons of vitamins and minerals.

The Keto diet is one of the most popular diets in the world at the moment and for good reason. This low carb high in good fats diet helps the body burn stored fat through a process called "ketosis", hence the name. The key to success is sticking with the diet because when you slip up, your body slips out of the ketosis state you've put it in. When it comes to sweet goodies this can be a little bit difficult. However, it doesn't have to be! Here are 6 smoothie combinations for you to try playing around with that will feel like you're cheating on your diet but are perfectly keto-friendly. 1. COCONUT CHOCOLATE DELIGHT Coconut is one of the best ingredients for a keto smoothie. It has those good fats your body needs in order for it to stay in ketosis. It tastes pretty good, but what tastes better than coconut? That's right - chocolate. Chocolate and coconut are a smoothie match made in heaven and can be used to make a Keto smoothie bowl recipe that acts as an alternative to chocolate pudding. The coconut gives the mixture a really smooth and creamy consistency and using cocoa powder is a really healthy way to get your chocolate fix for the day. If you want to be extra healthy, you can substitute the cocoa powder with a chocolate protein powder.

Scientists Katalin Kariko and Drew Weissman from Hungary and the United States respectively won the 2023 Nobel Prize in Physiology or Medicine for discoveries enabling the development of mRNA COVID-19 vaccines, the award-giving body said on Monday. The prize, among the most prestigious in the scientific world, is selected by the Nobel Assembly of Sweden's Karolinska Institute medical university and also comes with 11 million Swedish crowns (about £823,500). "The 2023 Nobel Prize in Physiology or Medicine has been awarded to Katalin Karikó and Drew Weissman for their discoveries concerning nucleoside base modifications that enabled the development of effective mRNA vaccines against COVID-19," the body said. Kariko was senior vice president and head of RNA protein replacement at BioNTech until 2022 and has since acted as an adviser to the company. She is also a professor at the University of Szeged in Hungary and adjunct professor at the University of Pennsylvania's Perelman School of Medicine. Weissman is professor in vaccine research at the Perelman School.

US-based BioMarin Pharmaceutical has selected a new drug development candidate, BMN 250, for the treatment of Sanfilippo B syndrome or Mucopolysaccharidosis type IIIB (MPS IIIB).

You need to know some facts about milk which can be helpful for your health.Most importantly milk is so much balanced having many number of nutrients. 1.Many nutritional components in milk such as Carbohydrate,Proteins,Vitamins,Minerals,Fats etc. 2.The protein and carbohydrate of milk gives us so much energy.

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