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Preclinical imaging and biological irradiation are pivotal in the development of new animal models, significantly enhancing the precision and depth of research. 1. Preclinical Imaging Preclinical in vivo imaging techniques allow researchers to non-invasively visualize and monitor disease progression, anatomical changes, and molecular processes in real-time, providing critical insights into the pathophysiology of various conditions. This capability enables the creation of more accurate and representative animal models of human diseases, such as cancer, neurological disorders, and cardiovascular diseases.